Main Points:
– Co-lead authors Xiaozhu Zhang and Briana Van Treeck from UC Berkeley developed an approach using RNAs with an avian R2 retroelement encoding and a transgene up to 4 kb.
– The R2 protein identifies the target site, creates a nick on one strand at a specific spot, and kick-starts DNA synthesis for stable transgene integration.
Author’s Take:
Zhang and Van Treeck’s groundbreaking approach of RNAs encoding an avian R2 retroelement opens up possibilities for precise transgene insertion up to 4 kb in length. This innovative technique marks a significant advancement in genetic engineering, promising more accurate and stable transgene integration processes.
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